albicans proteome to the components of the AAF fraction. Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. The loss of the correct cell shape and cell wall integrity was visualized by both the TEM and SEM techniques.

Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM). It was observed that, after the AAF treatment, the mitochondrial DNA migrated towards the nuclear DNA, whereupon both merged into a single nuclear structure, which preceded the apoptotic process. Staining of the cells with acridine orange revealed a change in the pH of the AAF-treated cells. Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other. The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.

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